Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Schematic showing the predicted protein structure of MSP2 and the derivation of the recombinant proteins. Filled blocks indicate sequences that are conserved among all P. falciparum isolates. Hatched blocks indicate dimorphic sequences which differ between A family and B family proteins but which are conserved within families. Checkered blocks indicate the highly polymorphic central region of the molecule, which contains tandemly repeated amino acid sequences. The recombinant proteins representing the dimorphic sequences (Di-A and Di-B) comprise the N-terminal dimorphic sequence fused to the C-terminal dimorphic sequence with exclusion of the intervening polymorphic sequence.

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: Recombinant, Sequencing

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Median antibody titers after three immunizations of C57BL/6 mice with recombinant MSP2 and MSP1 19 proteins

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: Recombinant

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Total IgG and IgG subclass antibody responses to MSP119 and MSP2 (serogroup A) proteins in C57BL/6 mice. Median midpoint titers of IgG and IgG subclasses (measured by ELISA) are shown for sera (six mice per group) collected prior to immunization and 2 weeks after each immunization with GST, MSP2, or MSP119 proteins. Horizontal dotted lines indicate a titer of 105. (a) Total IgG titers. Sera were tested against the same protein that was used for immunization. (b) IgG subclass titers to GST alone. Geometric mean titers are shown for all groups of mice combined (n = 36). (c to h) IgG subclass titers for mice immunized with different recombinant proteins. Sera were tested against the same protein that was used for immunization. (c) Full-length MSP2-His6 (MSP2A); (d) Di-A-GST; (e) Poly-A-GST; (f) Con-N; (g) Con-C-GST; (h) unrelated antigen-GST (MSP119).

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Lymphoproliferative responses a of splenocytes from immunized C57BL/6 mice to recombinant protein and synthetic peptide antigens

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: Recombinant

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Conserved C terminus of MSP2 induces rapid secretion of IL-6 and IL-10 by spleen cells from immunized C57BL/6 mice. Four weeks after the third immunization, spleen cells from mice (six per group) immunized with either MSP2A, Con-C, or MSP119 were collected and restimulated in vitro for 3 or 5 days with either the original immunizing antigen (a, b) or the C8 peptide (c, d). Culture supernatants were assayed by a cytokine bead assay. Data are presented as geometric mean cytokine concentrations (pg/ml). Cytokine values for cells cultured with GST alone (for a and b) or for cells cultured with an irrelevant peptide (for c and d) have been subtracted. ND, no detectable cytokine.

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: In Vitro, Cell Culture

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: B cells from IFN-γ-deficient and IL-10-deficient mice fail to class switch to IgG2b. Wild-type (WT), IFN-γ−/− (a), and IL-10−/− (b) mice (six per group) were immunized with MSP119, MSP2A, or Con-C, and median midpoint titers of IgG1 and IgG2b were measured by ELISA 2 weeks after the final immunization. The statistical significance of differences between wild-type and cytokine-deficient mice was determined using nonparametric (Wilcoxon signed rank) tests and is indicated. *, P < 0.05; **, P < 0.005.

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques: Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: The C8 peptide of MSP2 drives class switching of the anti-GST antibody response to IgG2b. Mice (four per group) immunized with (a) MSP119-GST, (b) MSP2A-GST, or (c) Con-C-GST were tested for antibodies to GST alone. Data represent median midpoint titers of each IgG subclass.

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques:

Journal:

Article Title: Epitope-Specific Regulation of Immunoglobulin Class Switching in Mice Immunized with Malarial Merozoite Surface Proteins

doi: 10.1128/IAI.73.12.8119-8129.2005

Figure Lengend Snippet: Peptide C8 drives class switching of the antibody response to MSP119, GST, and BSA to IgG2b. Ratios of midpoint titers of IgG2b to IgG1 antibodies to (a) MSP1119 (b) GST, and (c) BSA in the sera of C57BL/6 mice (four per group) immunized with (a, b) MSP119-GST, MSP119-GST conjugated with peptide C8 (C8-MSP119-GST), MSP119-GST conjugated with an irrelevant peptide (L-MS119-GST), (c) BSA alone, BSA conjugated to peptide L (L-BSA), or BSA conjugated to C8 (C8-BSA). Each bar represents the IgG2b/IgG1 ratio for serum from one mouse. * indicates a serum where the ratio was 1.0.

Article Snippet: Peptides (C8 and L) were conjugated to bovine serum albumin (BSA; Pierce Biotechnology, Inc., Tattenhall, United Kingdom) and to GST-MSP1 19 activated with sulfo-MBS ( m -maleimidobenzoyl- N -hydroxysulfosuccinimide ester) (Pierce Biotechnology, Inc.) according to the manufacturer's protocol. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Immunizing antigen Median midpoint titer (10 5 ) (95% CI) a Mean IgG1/IgG2b ratio b P (MSP2 vs MSP1 19 ) Total IgG IgG1 IgG2a IgG2b IgG3 MSP1 19 8.34 (2.19) 5.02 (1.97) 0.08 (0.41) 3.89 (0.52) 0.05 (0.13) 2.89 Serogroup A Full length (MSP2A) 9.60 (1.79) 6.34 (1.66) 0.83 (0.44) 2.95 (0.37) 0.43 (0.18) 4.69 0.008 Di-A 17.8 (3.57) 2.55 (1.43) 0.18 (0.09) 12.0 (3.09) 0.11 (0.08) 0.32 0.007 Poly-A 0.64 (0.30) 0.19 (0.12) 0.04 (0.02) 0.47 (0.12) 0.03 (0.02) 0.51 0.011 Serogroup B Full length (MSP2B) 19.3 (4.55) 11.8 (2.58) 0.78 (0.41) 3.91 (1.38) 0.51 (0.13) 5.92 0.014 Di-B 3.84 (1.89) 0.76 (0.47) 0.04 (0.01) 2.50 (1.25) 0.03 (0.03) 0.34 0.009 Poly-B 5.5 (0.63) 1.11 (0.88) 0.07 (0.05) 4.37 (1.25) 0.03 (0.01) 0.44 0.008 Con-C 2.61 (0.28) 0.18 (0.12) 0.08 (0.06) 2.22 (0.63) 0.06 (0.03) 0.18 0.006 Con-N 0.64 (0.11) 0.45 (0.08) 0.02 (0.01) 0.42 (0.10) 0.01 (0.01) 1.46 0.064 Open in a separate window a There were six mice per group.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis

doi: 10.3389/fvets.2021.698800

Figure Lengend Snippet: Immune markers used for immunohistochemistry.

Article Snippet: CD4 , Mouse anti bovine CD4 (monoclonal) , 1/30 , BIO-RAD , NA , Goat vs. mouse (1/200) , RT, 1 h , BIO-RAD , PBS.

Techniques: Immunohistochemistry, Incubation

Journal: Chinese medicine

Article Title: Shen-Bai-Jie-Du decoction suppresses the progression of colorectal adenoma to carcinoma through regulating gut microbiota and short-chain fatty acids.

doi: 10.1186/s13020-024-01019-4

Figure Lengend Snippet: Fig. 9 The schematic diagram of the mechanism of SBJDD. SBJDD may exert its inhibitory effects on colorectal adenoma carcinogenesis by regulating gut microbiota, promoting the production of SCFAs, activating G protein-coupled receptors GPR43, GPR41 and GPR109a, inhibiting histone deacetylases HDAC1 and HDAC3, inducing M2-like macrophage polarization, reducing intestinal inflammation, inhibiting cancer cell proliferation and restoring intestinal barrier function

Article Snippet: Anti-GAPDH (no. 2118S), anti-β-Tubulin (no. 2146S) and Mouse Reactive M1 vs M2 Macrophage IHC Antibody Sampler Kit (no. 97624 T) were obtained from Cell Signaling Technology (Beverly, USA).

Techniques:

Journal: Parasites & Vectors

Article Title: Histopathological and immunohistochemical characterisation of hepatic granulomas in Leishmania donovani -infected BALB/c mice: a time-course study

doi: 10.1186/s13071-018-2624-z

Figure Lengend Snippet: Antibodies and reagents used for immunohistochemistry

Article Snippet: iNOS , Rabbit vs Mouse iNOS (polyclonal) , 1/500 , Merck Millipore (ABN26) (Watford, Hertfordshire, UK) , None (ZSF samples) , Goat vs Rabbit (1/1000) , TBS.

Techniques:

Journal: Parasites & Vectors

Article Title: Histopathological and immunohistochemical characterisation of hepatic granulomas in Leishmania donovani -infected BALB/c mice: a time-course study

doi: 10.1186/s13071-018-2624-z

Figure Lengend Snippet: Immunohistochemical detection of macrophages (F4/80+) within hepatic granulomas at 15 dpi ( a ), 35 dpi ( b ) and 63 dpi ( c ). d Percentage of immunostained area for F4/80 at 15, 35 and 63 dpi within immature (I), mature (M) and clear (C) granulomas. Immunohistochemical detection of iNOS+ cells at 15 dpi ( e ), 35 dpi ( f ) and 63 dpi ( g ). h Percentage of immunostained area for iNOS at 15, 35 and 63 dpi within immature (I), mature (M) and clear (C) granulomas. * P < 0.05. Scale-bars : 50 μm

Article Snippet: iNOS , Rabbit vs Mouse iNOS (polyclonal) , 1/500 , Merck Millipore (ABN26) (Watford, Hertfordshire, UK) , None (ZSF samples) , Goat vs Rabbit (1/1000) , TBS.

Techniques: Immunohistochemical staining